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Thyroid Hormone Testing in Dogs

Thyroid Hormone Testing in Dogs

It is very important that diagnostic testing that pertains to hormones be done accurately in order to ensure the health of your pet. Today, our Citrus Heights vets share some important information about thyroid hormone testing for dogs including how it's done and what the common types are.

What exactly is the thyroid?

The thyroid gland, which is located near the trachea, produces thyroxine (T4), a major thyroid hormone. Thyroid hormones have far-reaching effects on the body by regulating metabolic rate. The pituitary gland, located at the base of the brain, regulates thyroid gland function with a hormone called TSH (thyroid stimulating hormone).

Why is thyroid testing for dogs important?

A thyroid test is a blood test that evaluates the thyroid gland's function. It is recommended in any sick animal and is frequently used as a screening test for underlying illness or disease. If the thyroid test for your pet comes back normal it means that their hormone levels are as expected and the thyroid glands don't have any issues.

If the animal tends to have excessive bleeding, extra care should be taken after obtaining the sample to ensure no hemorrhaging from the site where the sample was obtained.

How is thyroid testing completed? 

A thyroid test requires drawing a blood sample, placing it in a special glass tube, and separating it into two parts: serum and blood clot. The blood clot is not needed for anything further and will be disposed of while the serum is used for testing. Some veterinary hospitals can perform thyroid tests in-house, but most rely on outside laboratories.

If performed at the veterinary hospital, a thyroid test usually takes about 40–60 minutes. If given to an outside laboratory, you can expect the results within 1–2 days.

Most dogs do not require sedation or anesthesia. Some dogs, however, dislike needles and may require anesthesia.

What are the typical types of thyroid hormone testing?

The following are some of the most common thyroid tests done for dogs.

T4 & T3

Total T4 (Thyroxine) and Total T3 (Triiodothyronine) testing can be used to screen for hypothyroidism in dogs. Unexpectedly high levels of either hormone may be indicative of autoantibodies, and T3 and T4 concentrations can be influenced by a variety of factors including medications, disease states, and nutrition.

Free T4 by lmmulite or by Equilibrium Dialysis

A valid assay for measuring free T4 (FT4) can be used to distinguish true hypothyroidism from euthyroid sick condition. The non-protein bound thyroxine, FT4, is found in lower concentrations in the blood than total T4. A method should be used to separate the protein-bound hormone from the free (unbound) hormone for accurate FT4 testing.

The Equilibrium Dialysis (ED) method is the gold standard test for dogs, requiring an overnight incubation in buffer and dialysis cells to separate bound T4 from free T4. The Immulite method is less expensive and faster than the ED method, producing results comparable to dialysis. Thyroid supplementation should be monitored using FT4 in any dog known or suspected to have thyroid autoantibodies, as these tests remove the autoantibody effects.

Thyroglobulin Autoantibody (TgAA) Test

The TgAA test is a canine-specific test for detecting autoimmune thyroiditis. For a more accurate diagnosis, it should be used in conjunction with other thyroid tests. Thyroglobulin autoantibodies are involved in the synthesis of T4 and T3.

TSH measurement

The endogenous thyroid stimulating hormone (TSH) can be measured in dogs. High levels of endogenous thyroid-stimulating hormone levels suggest hypothyroidism, but normal or low endogenous thyroid-stimulating hormone levels in dogs do not necessarily rule it out. This test should be used in conjunction with other thyroid tests to make a diagnosis.

Note: The advice provided in this post is intended for informational purposes and does not constitute medical advice regarding pets. For an accurate diagnosis of your pet's condition, please make an appointment with your vet.

Are you concerned that your dog may be having issues affecting their thyroid or other concern that requires diagnostic testing? Don't hesitate to contact our Citrus Heights vets to schedule an appointment today.

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Family Friends Veterinary Hospital is happy to accept new patients! Our vets are passionate about improving the health of Citrus Heights companion animals. Get in touch today to book your pet's first appointment.

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